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Overexpression or deletion of ergosterol biosynthesis genes alters doubling time, response to stress agents, and drug susceptibility in saccharomyces cerevisiae

Risks rise sharply with unregulated providers or home use

10.1378/chest.100.5.1397 Chest

BRET measurements were carried out in HEK293T cells co-expressing Nluc-fused mas-GsBP1, mas-GsBP2, mas-KB1753 (in D, E and F), mas-GRK RH (in G), or mas-PRG RH (in H), with either wild-type (WT) or constitutively active (QL) mutants of Gi3 (in D), Go (in E), Gz (in F), Gq (in G), or G13 (in H) fused to a YFP, as indicated

If possible, reduce the dilution factor of the pretreatment or increase the sample volume to increase the concentration of the sample above the measuring range